Serine/threonine-protein kinase Nek1, Santa Cruz Biotechnology, sc-398813

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Serine/threonine-protein kinase Nek1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human Serine/threonine-protein kinase Nek1 encoded by NEK1

The collection of antibodies was tested using HeLa WT and NEK1 KO cells in the applications WB (WT and KO cell lysates/100 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:250 antibody dilution), sc-398813 did not reveal any clearly defined bands in the WT cell lysate, and no corresponding signal was observed in the KO cell lysate. The signal appears very weak and diffuse, and due to the low quality of the gel, no reliable conclusions regarding protein detection or size can be drawn. No band was detected following IP (1 µg Ab used). In IF (1:300 ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.