NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, Abcam, ab192022

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 encoded by NDUFS2.

The collection of antibodies was tested using HAP1 WT and NDUFS2 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:2000 Ab dilution) ab192022 detected an intense band of appr. 46 kDa in WT cell lysate not observed in KO cell lysate. A band of higher molecular weight was observed in both genotypes. No band was observed following IP (2 µg Ab used). In IF (1:800 ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.