Malic enzyme 1, Aviva Systems Biology, ARP74638_P050

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Malic Enzyme 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Malic Enzyme 1 encoded by ME1.

The collection of antibodies was tested using HAP1 WT and ME1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) ARP74638_P050 detected an intense band of appr. 63 kDa in WT cell lysate, with a faint corresponding band observed in KO cell lysate. Several bands of both higher and lower molecular weight were observed in both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:250 Ab dilution), a more intense staining of WT cells was observed compared to KO cells also showing background staining.

We highly encourage other scientists to share their observations.