Malic enzyme 1, Aviva Systems Biology, ARP32794_P050

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Malic Enzyme 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Malic Enzyme 1 encoded by ME1.

The collection of antibodies was tested using HAP1 WT and ME1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) ARP32794_P050 detected a band of appr. 60 kDa in both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:250 Ab dilution), a more intense staining of WT cells was observed compared to KO cells also showing background staining.

We highly encourage other scientists to share their observations.