MDH1, Developmental Studies Hybridoma Bank, CPTC-MDH1-1

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Malate dehydrogenase 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Malate dehydrogenase 1 encoded by MDH1.

The collection of antibodies was tested using HAP1 WT and MDH1 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:68 Ab dilution) CPTC-MDH1-1 detected multiple bands (>10), none of which were specific to WT cell lysate. No band was detected following IP (2 µg Ab used). In IF (1:500 Ab dilution), comparable staining intensity was observed in both KO and WT cells.

We highly encourage other scientists to share their observations.