MDH1, Aviva Systems Biology, ARP48284_T100

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Malate dehydrogenase 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Malate dehydrogenase 1 encoded by MDH1.

The collection of antibodies was tested using HAP1 WT and MDH1 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:250 Ab dilution) ARP48284_T100 detected multiple bands (>10), one faint band was specific to WT cell lysate observed at appr. 37 kDa not seen in KO cell lysate. Several of the other bands has stronger intensity. No band was detected following IP (2 µg Ab used). In IF (1:500 Ab dilution), comparable staining intensity was observed in both KO and WT cells.

We highly encourage other scientists to share their observations.