MDH1, ABclonal, A7563

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Malate dehydrogenase 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Malate dehydrogenase 1 encoded by MDH1.

The collection of antibodies was tested using HAP1 WT and MDH1 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) A7563 detected a band of appr. 37 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. No band was detected following IP (2 µg Ab used). In IF (1:1000 Ab dilution), a weak staining was observed in WT cells with some background staining in KO cells.

We highly encourage other scientists to share their observations.