Matrin-3, Thermo Fisher Scientific, MA5-34642

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Matrin-3 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 4 commercial research antibodies against human Matrin-3 encoded by MATR3.

The collection of antibodies was tested using HAP1 WT and MATR3 KO cells in the applications WB (WT and KO cell lysates/35 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) MA5-34642 detected a band of appr. 120 kDa in WT cell lysate not observed in KO cell lysate. No band was detected following IP (2 µg Ab used). In IF (1:1000 ab dilution) WT cells showed more intense staining compared to the weaker staining observed in KO cells.

We highly encourage other scientists to share their observations.