MAPK 1, Abcam, ab32081

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Mitogen-activated protein kinase 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Mitogen-activated protein kinase 1 encoded by MAPK1.

The collection of antibodies was tested using HeLa WT and MAPK1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) ab32081 detected a band of appr. 40 kDa in WT cell lysate not observed in KO cell lysate. Bands of both higher and lower molecular weight was observed in KO cell lysate only. Only a band corresponding to the heavy chain was detected following IP (2 µg Ab used). In IF (1:400 Ab dilution) a clear intense staining was observed in WT cells with some background staining in KO cells.

We highly encourage other scientists to share their observations.