MAPK 1, Thermo Fisher Scientific, MA5-32025

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Mitogen-activated protein kinase 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Mitogen-activated protein kinase 1 encoded by MAPK1.

The collection of antibodies was tested using HeLa WT and MAPK1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) MA5-32025 detected a single clear band of appr. 40 kDa in WT cell lysate not observed in KO cell lysate. No band was detected following IP (2 µg Ab used). In IF (1:1000 Ab dilution), a clear intense staining was observed in WT cells with little to none background staining in KO cells.

We highly encourage other scientists to share their observations.