Leucine-rich repeat kinase 2, Thermo Fisher Scientific, PA5-85363

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Leucine-rich repeat kinase 2 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Leucine-rich repeat kinase 2 encoded by LRRK2.

The collection of antibodies was tested using A549 WT and LRRK2 KO cells in the applications WB (WT and KO cell lysates/25 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) PA5-85363 detected multiple bands (>5) none of which were specific to WT cell lysate. Only a faint band was detected following IP (2 µg Ab used). In IF (1:500 ab dilution) WT cells showed only slightly stronger staining intensity compared to KO cells.

We highly encourage other scientists to share their observations.