Leucine-rich repeat kinase 2, Abcam, ab133474

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Leucine-rich repeat kinase 2 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Leucine-rich repeat kinase 2 encoded by LRRK2.

The collection of antibodies was tested using A549 WT and LRRK2 KO cells in the applications WB (WT and KO cell lysates/25 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:10000 Ab dilution) ab133474 detected a band of appr. 245 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. No band was detected following IP (2 µg Ab used). In IF (1:100 Ab dilution) KO cells showed stronger staining intensity compared to WT cells.

We highly encourage other scientists to share their observations.