LRP-1, Cell Signaling Technology, 64099

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Prolow-density lipoprotein receptor-related protein 1 (LRP-1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human LRP-1 encoded by LRP1.

The collection of antibodies was tested using HAP1 WT and LRP1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) 64099 detected two bands of appr. 80 kDa and 500 kDa corresponding to the alpha- and beta subunit, receptively, in WT cell lysate not observed in KO cell lysate. A band at appr. 80 kDa was also detected following IP (2 µg Ab used). This band was as intense as the corresponding band in input extract (4% of starting material). A band was detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from the cell lysate. In IF (1:100 ab dilution) a more intense staining intensity was observed in WT cells compared to KO cells showing some background.

We highly encourage other scientists to share their observations.