MDA-5, Proteintech, 21775-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Interferon-induced helicase C domain-containing protein 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 9 commercial research antibodies against human Interferon-induced helicase C domain-containing protein 1 encoded by IFIH1.

The collection of antibodies was tested using DMS53 ctrl and IFIH1 KD cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference). siRNA technology was performed to knockdown (KD) the target gene.

In WB (1:2000 Ab dilution) 21775-1-AP detected a band of appr. 120 kDa in Ctrl cell lysate with no corresponding band observed in KD cell lysate. Several bands of lower molecular weight were observed in both genotypes. A band was also detected following IP (2 µg Ab used). This band was more intense than the corresponding band in input extract (4% of starting material). Band was not detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does completely deplete the antigen from the cell lysate. In IF (1:600 Ab dilution), a clearer staining intensity was observed in Ctrl cells compared to KD cells showing background staining.

We highly encourage other scientists to share their observations.