Huntingtin, Thermo Fisher Scientific, MA5-41256

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Huntingtin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 20 commercial research antibodies against human Huntingtin encoded by HTT.

The collection of antibodies was tested using DMS35 WT and HTT KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:2000 Ab dilution) MA5-41256 detected a band of appr. 350 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A faint band at lower molecular weight was detected in both genotypes. Only a faint band was detected following IP (2 µg Ab used). In IF (1:1000 Ab dilution), WT cells display higher signal intensity compared to KO cells, which show low or background-level staining.

We highly encourage other scientists to share their observations.