Huntingtin, Thermo Fisher Scientific, MA5-16703

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Huntingtin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 20 commercial research antibodies against human Huntingtin encoded by HTT.

The collection of antibodies was tested using DMS35 WT and HTT KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 antibody dilution), MA5-16703 detected a strong double band at ~279 kDa in WT cell lysate, with no corresponding bands in KO cell lysate. A faint band at lower molecular weight (~60 kDa) was detected in both genotypes. A faint double band was detected following IP (2 µg Ab used). In IF (1:1000 Ab dilution), comparable staining intensity was observed in both genotypes.

We highly encourage other scientists to share their observations.