Huntingtin, Thermo Fisher Scientific, 710695

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Huntingtin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 20 commercial research antibodies against human Huntingtin encoded by HTT.

The collection of antibodies was tested using DMS35 WT and HTT KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 Ab dilution) 710695 detected a band of appr. 75 kDa in both genotypes, not corresponding with the predicted band size. No band was detected following IP (2 µg Ab used). In IF (1:500 Ab dilution), comparable staining intensity was observed in both genotypes.

We highly encourage other scientists to share their observations.