Huntingtin, GeneTex, GTX638832

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Huntingtin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 20 commercial research antibodies against human Huntingtin encoded by HTT.

The collection of antibodies was tested using DMS35 WT and HTT KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference). Furthermore, was this antibody evaluated in HEK 293T and HAP1in the application WB (WT and KO cell lysates/30 µg protein).

In WB, GTX638832 detected a band at ~350 kDa in WT cell lysate with no corresponding band in KO cell lysate at 1:500 antibody dilution; two additional bands of lower molecular weight were also observed in WT lysate. At 1:1000 dilution, GTX638832 detected a double band at ~279 kDa in WT lysates from DMS53, HEK293T, and HAP1 cells, with the signal strongly reduced or absent in the corresponding KO lysates. No band was detected following IP (2 µg Ab used). In IF (1:500 Ab dilution) WT cells display higher signal intensity compared to KO cells, which show background-level staining.

We highly encourage other scientists to share their observations.