Huntingtin, GeneTex, GTX132433

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Huntingtin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 20 commercial research antibodies against human Huntingtin encoded by HTT.

The collection of antibodies was tested using DMS35 WT and HTT KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) GTX132433 detected a band of appr. 350 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Multiple bands (>8) were observed in both genotypes. A band was detected following IP (2 ug Ab used), this band was less intense than corresponding bands in input extract (4% of starting material) and immunodepleted extract (4% of total unbound fraction). In IF (1:500 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.