Huntingtin, ABclonal, A19064

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Huntingtin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 20 commercial research antibodies against human Huntingtin encoded by HTT.

The collection of antibodies was tested using DMS35 WT and HTT KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) A19064 detected a band of appr. 350 kDa in WT cell lysate with a faint corresponding band in KO cell lysate: additional bands of higher intensity were also observed in both genotypes. A band was also detected following IP (2 µg Ab used). This band was equally intense compared to input extract (4% of starting material). Band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from the cell lysate. In IF (1:600 Ab dilution) clearer staining was observed in WT cells, with some background signal in KO cells.

We highly encourage other scientists to share their observations.