hnRNP A1, Thermo Fisher Scientific, MA5-35132

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human hnRNP A1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human hnRNP A1 encoded by HNRNPA1.

The collection of antibodies was tested using HEK293T WT and HNRNPA1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) MA5-35132 detected a double band at appr. 35 kDa in WT lysate not observed in KO lysate. Immunoprecipitation using 2 µg antibody did not yield a detectable band for the protein of interest; only the antibody heavy chain was detected. In IF (1:300 Ab dilution), WT cells showed more intense staining compared to KO cells.

We highly encourage other scientists to share their observations.