hnRNP A1, GeneTex, GTX106208

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human hnRNP A1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human hnRNP A1 encoded by HNRNPA1

The collection of antibodies was tested using HEK293T WT and HNRNPA1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) GTX106208 detected an intense band at appr. 38 kDa in WT lysate with no corresponding band observed in KO cell lysate. Background bands of higher and lower molecular weight was observed for both genotypes. Immunoprecipitation using 2 µg antibody did not yield a detectable band for the protein of interest; only the antibody heavy chain was detected. In IF (1:500 Ab dilution), comparable staining intensities were observed for both KO and WT cells.

We highly encourage other scientists to share their observations.