hnRNP A1, Cell Signaling Technology, 8443

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human hnRNP A1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human hnRNP A1 encoded by HNRNPA1.

The collection of antibodies was tested using HEK293T WT and HNRNPA1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) 8443 detected a band of appr. 38 kDa in WT lysate with no corresponding band observed in KO lysate. Immunoprecipitation using 2 µg antibody did not yield a detectable band for the protein of interest; only the antibody heavy chain was detected. In IF (1:500 Ab dilution), WT cells showed more intense staining compared to KO cells showing some background staining.

We highly encourage other scientists to share their observations.