Gelsolin, Santa-Cruz, sc-271001

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Gelsolin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human Gelsolin encoded by GSN.

The collection of antibodies was tested using U2OS WT and GSN KO cells in the applications WB (WT and KO cell lysates), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

WB was performed using lysates (100 µg protein) from WT and KO cells. WB with sc-271001 (antibody dilution 1:1000) produced no band. No band was detected following IP (1 µg Ab used). In IF (1:1000 Ab dilution) no signal was detected.

We highly encourage other scientists to share their observations.