Gelsolin, Bio-Techne, MAB8170

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Gelsolin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human Gelsolin encoded by GSN.

The collection of antibodies was tested using U2OS WT and GSN KO cells in the applications WB (WT and KO cell lysates), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference). Additionally, was this antibody tested using HeLa in Western blot.

WB was performed using lysates (50 µg protein) from WT and KO cells. WB with MAB8170 (antibody dilution 1:5000), produced a single band of appr. 80 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Lysate from HeLa cells (WT and KO) were prepared and tested in WB with MAB8170 (1:5000 Ab dilution), which detected a band of appr. 80 kDa in WT with no corresponding band in KO lysate. No band was detected following IP (1 µg Ab used). In IF (1:1000 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.