Gelsolin, Abcam, ab11081

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Gelsolin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human Gelsolin encoded by GSN.

The collection of antibodies was tested using U2OS WT and GSN KO cells in the applications WB (WT and KO cell lysates), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

WB was performed using lysates (50 µg protein) from WT and KO cells. WB with ab11081 (antibody dilution 1:200), produced a single band of appr. 80 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. A band was also detected following IP (1 µg Ab used), this band was as intense as the band in input extract (10 % of starting material) and immunodepleted extract (10 % of total unbound fraction), showing that the antibody does not deplete the antigen from the cell lysate. In IF (1:1000 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.