Gelsolin, Proteintech, 11644-2-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Gelsolin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human Gelsolin encoded by GSN.

The collection of antibodies was tested using U2OS WT and GSN KO cells in the applications WB (WT and KO cell lysates), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

WB was performed using lysates (50 µg protein) from WT and KO cells. WB with 11644-2-AP (antibody dilution 1:5000) produced a single band of appr. 80 kDa in WT lysate with no corresponding band observed in KO lysate. A band was also detected following IP (1 µg Ab used), this band was less intense than the band in input extract (10 % of starting material). A faint band was also detected in immunodepleted extract (10 % of total unbound fraction), showing that the antibody does not completely deplete the antigen from cell lysate. In IF (1:500 Ab dilution), WT cell exhibited a more intense staining compared to KO cell showing some background.

We highly encourage other scientists to share their observations.