Gelsolin, GeneTex, GTX114078

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Gelsolin and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 16 commercial research antibodies against human Gelsolin encoded by GSN.

The collection of antibodies was tested using U2OS WT and GSN KO cells in the applications WB (WT and KO cell lysates), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

WB was performed using lysates (25 µg protein) from WT and KO cells. WB with GTX114078 (antibody dilution 1:10000) produced a strong clean band in WT cell lysate with no corresponding band observed in KO cell lysate. A band was also detected following IP (1 µg Ab used), this band was slightly more intense than the band observed in Input extract (10 % of starting material). A band was also observed in immunodepleted extract (10 % of total unbound fraction) showing that the antibody does not completely deplete the antigen from cell lysate. In IF (1:1000 Ab dilution), WT cell exhibited slightly more intense staining compared to KO cell.

We highly encourage other scientists to share their observations.