GPNMB, Abcam, ab227695

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human GPNMB and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 20 commercial research antibodies against human GPNMB.

The collection of antibodies was tested using U-87 MG WT and GPNMB KD cells in the applications WB (WT and KD cell lysates/60 µg protein), IP (WT cell lysate) and IF (WT and KD cells) according to YCharOS standard protocols (see reference). siRNA technology was used to knockdown (KD) the target gene.

In WB (1:500 Antibody dilution) ab227695 detected a double band of appr. 80 and 100 kDa in control lysate not observed in KD lysate. No band was detected following IP (2 µg Ab used). In IF (1:500 Antibody dilution) control cells showed a stronger signal compared to KD cells, only showing some background.

We highly encourage other scientists to share their observations.