Galactosylceramidase, MilliporeSigma, MAB342

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human galactosylceramidase and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 6 commercial research antibodies against human galactosylceramidase encoded by GALC gene.

The collection of antibodies was tested using OCUM-1 WT and GALC KD cells in the applications WB (WT and KD cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KD cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) MAB342 detected no band of interest in either WT or KD cell lysate. No bands were detected following IP (2 µg Ab used). In IF (1:600 Ab dilution), KD cells showed more intense staining compared to WT cells.

We highly encourage other scientists to share their observations.