RNA-binding protein FUS, Thermo Fisher Scientific, MA5-32483

Our team has carried out an in-depth review of the published scientific literature to identify papers and reports using antibodies directed against the human RNA-binding protein Fused-in-Sarcoma, followed by extraction and evaluation of the available data to assess performance according to the pAbmAbs rating criteria.

YCharOS is conducting a large head-to-head study comparing 18 commercially available research antibodies against the human RNA-binding protein Fused-in-Sarcoma encoded by FUS.

The collection of antibodies was tested using HeLa WT and FUS KO cells in the applications WB (WT and KO cell lysates, 30 µg protein), IP (WT cell lysate), IF (WT and KO cells), and flow cytometry (WT and KO cells). Some antibodies were evaluated across multiple applications, whereas others were tested exclusively by flow cytometry, all according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) MA5-32483 detected a band of appr. 70 kDa in WT cell lysate not observed in KO cell lysate. No band was observed following IP (1 µg Ab used). In IF (1:1000 Ab dilution), comparable staining intensities was observed for both KO and WT cells. Flow cytometry using MA5-32483 (0.2 μg/mL antibody) showed nearly identical fluorescence intensity distributions for HeLa WT and FUS KO cells. Both populations were shifted relative to the secondary-only control, indicating substantial non specific staining and no detectable FUS dependent signal with this antibody.

We highly encourage other scientists to share their observations.