Polyphosphoinositide phosphatase, BioLegend, 861802

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Polyphosphoinositide phosphatase and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 5 commercial research antibodies against human Polyphosphoinositide phosphatase encoded by FIG4.

The collection of antibodies was tested using HAP1 WT and FIG4 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) 861802 detected a weak band of appr. 100 kDa in WT cell lysate not observed in KO cell lysate. No band was detected following IP (2 µg Ab used). In IF (1:500 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.