Polyphosphoinositide phosphatase, Addgene, 192037

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Polyphosphoinositide phosphatase and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 5 commercial research antibodies against human Polyphosphoinositide phosphatase encoded by FIG4.

The collection of antibodies was tested using HAP1 WT and FIG4 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:200 Ab dilution) 192037 detected a band of appr. 100 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. In both genotypes a faint background band of lower molecular weight was observed in lysates. No band was detected following IP (2 µg Ab used). In IF (1:1000 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.