Endothelin-converting enzyme 1, Abcam, ab71829

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Endothelin-converting enzyme 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing six commercial research antibodies against human Endothelin-converting enzyme 1 encoded by ECE1

The collection of antibodies was tested using U-87MG WT and ECE1 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) ab71829 produced multiple bands none of which were specific to WT cell lysate. No band was detected following IP (2 µg Ab used). In IF (1:1000 Ab dilution) a KO cell showed strong staining intensity, compared to other cells. Overall comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.