DYRK1A, Thermo Fisher Scientific, PA5-95499

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human DYRK1A (dual-specificity tyrosine phosphorylation-regulated kinase 1A) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing seven commercial research antibodies against human DYRK1A.

The collection of antibodies was tested using 5637 WT and DYRK1A KD cells in the applications WB (WT and KD cell lysates/60 µg protein) and IP (WT cell lysate) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) PA5-95499 detected a band of appr. 85 kDa in both WT and KD cell lysates. No band was observed following IP (2 µg Ab used).

We highly encourage other scientists to share their observations.