DYRK1A, R&D Systems, AF5407

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human DYRK1A (dual-specificity tyrosine phosphorylation-regulated kinase 1A) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing seven commercial research antibodies against human DYRK1A.

The collection of antibodies was tested using 5637 WT and DYRK1A KD cells in the applications WB (WT and KD cell lysates/60 µg protein) and IP (WT cell lysate) according to YCharOS standard protocols (see reference).

In WB (1:100 Ab dilution) AF5407 detected a faint band of appr. 85 kDa in WT cell lysate, with no corresponding band observed in KD cell lysate. Background bands of higher molecular weight were observed in lysates of both genotypes. A band was also detected following IP (2 µg Ab used). This band was more intense than the corresponding band in input extract (4% of starting material). A faint band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from the cell lysate.

We highly encourage other scientists to share their observations.