Dynamin-1, Sigma, MABT188

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Dynamin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human Dynamin-1 encoded by DNM1.

The collection of antibodies was tested using U2OS WT and DNM1 KO cells in the applications WB (WT and KO cell lysates/130 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) MABT188 detected a band of appr. 97 kDa observed both in WT and KO cell lysate. Bands of stronger intensity of both higher and lower molecular weight was observed in both genotypes. A band was also detected following IP (1 µg Ab used). This band was markedly more intense than the corresponding band in input extract (10% of starting material). A very faint band was also detected in immunodepleted extract (10% of total unbound fraction). In IF (1:1000 Ab dilution), a clear staining was observed in WT cells with some background staining in KO cells.

We highly encourage other scientists to share their observations.