Dynamin-1, Thermo Fisher Scientific, MA5-34957

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Dynamin-1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human Dynamin-1 encoded by DNM1.

The collection of antibodies was tested using U2OS WT and DNM1 KO cells in the applications WB (WT and KO cell lysates/130 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) MA5-34957 detected a band of appr. 97 kDa in both WT and KO cell lysate. Bands of lower molecular weight was also observed. No band was observed following IP (1 µg Ab used). In IF (1:2000 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.