Dystroglycan1, Thermo Fisher Scientific, PA5-28179

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Dystroglycan 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Dystroglycan 1encoded by DAG1. Dystroglycan 1 is cleaved into the following 2 chains: Alpha-dystroglycan and Beta-dystroglycan. The antibodies tested has specificity for either one of the chains or both.

The collection of antibodies against Beta-dystroglycan was tested using A431WT and DAG1 KO cells in the applications WB (WT and KO cell lysates/90 µg protein) and IF (WT and KO cells), according to YCharOS standard protocols (see reference).

The collection of antibodies against Alfa-dystroglycan was tested using HAP1 WT and DAG1 KO cells in the applications WB (WT and KO cell lysates/100 µg protein) and IP (WT cell lysate), according to YCharOS standard protocols (see reference).

PA5-28179 has specificity for both Alpha-Dystroglycan and Beta-Dystroglycan. In WB (1:2000 Ab dilution) PA5-28179 detected no protein of interest. Several bands of lower molecular weight were observed for both genotypes. Furthermore, PA5-28179 was also tested in WB (1:2000 Ab dilution) on HAP1 culture media. No protein of interest was detected. No band was detected following IP (2 µg Ab used). In IF (1:2000 Ab dilution), comparable staining intensities were observed in both KO and WT cells.

We highly encourage other scientists to share their observations.