Cathepsin B, Abcam, ab58802

YCharOS previously conducted a large head-to-head study comparing commercial research antibodies against human Cathepsin B, encoded by CTSB. The antibodies were evaluated based on published scientific literature and experimental data according to the pAbmAbs rating criteria.

In the previous study, this antibody was evaluated by YCharOS using HAP1 WT and CTSB KO cells in Western blot (WB), following YCharOS standard protocols (see reference).

Building on these previous findings, the antibody was further evaluated by YCharOS in immunofluorescence (IF) using HAP1 WT and CTSB KO cells and in IP using HAP1 WT cell lysate. At an antibody dilution of 1:500, a more diffuse staining was observed in WT cells compared to KO showing background staining in IF. A band was detected following IP (2 µg Ab used) targeting mature Cathepsin B, this band had same intensity compared to band in input extract (4% of starting material) and immunodepleted extract (4% of total unbound fraction), showing that the antibody does not fully deplete the antigen from cell lysate.

We highly encourage other scientists to share their experience

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Cathepsin B and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 13 commercial research antibodies against human Cathepsin B encoded by CTSB.

The collection of antibodies was tested using HAP1 WT and CTSB KO cells in the application WB (WT and KO cell lysates/30 µg protein), according to YCharOS standard protocols (see reference).

In WB (1:200 Ab dilution) ab58802 detected a band of appr. 38 kDa in both genotypes. Furthermore, bands of higher and stronger intensity were observed for both WT and KO cell lysates.

We highly encourage other scientists to share their observations.