CSNK2A1, Cell Signaling Technology, 2656

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Casein kinase II subunit alpha and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Casein kinase II subunit alpha encoded by CSNK2A1.

The collection of antibodies was tested using HAP1 WT and CSNK2A1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) 2656 detected a band of appr. 45 kDa in WT cell lysate with no corresponding band in KO cell lysate. Several bands of both higher and lower molecular weight were observed for both genotypes. No band other than the antibody heavy chain was observed following IP (2 µg Ab used). In IF (1:30 Ab dilution) a clear staining was observed in WT cells with some background staining in KO cells.

We highly encourage other scientists to share their observations.