CSNK2A1, Abcam, ab236664

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Casein kinase II subunit alpha and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Casein kinase II subunit alpha encoded by CSNK2A1.

The collection of antibodies was tested using HAP1 WT and CSNK2A1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) ab236664 detected a band of appr. 45 kDa in WT cell lysate with no corresponding band in KO cell lysate. A band of higher molecular weight was detected in both genotypes. A band was also detected following IP (2 µg Ab used). This band was more intense than the corresponding band in input extract (4% of starting material). Only a faint band was detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does almost deplete the antigen from the cell lysate. In IF (1:100 Ab dilution) a more intense staining of WT cells was observed with some background in KO cells.

We highly encourage other scientists to share their observations.