CSNK1A1, Proteintech, 68434-1-Ig

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Casein kinase I isoform alpha and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Casein kinase I isoform alpha encoded by CSNK1A1.

The collection of antibodies was tested using HCT 116 WT and CSNK1A1 KD cells in the applications WB (WT and KD cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KD cells) according to YCharOS standard protocols (see reference).

In WB (1:3000 Ab dilution) 68434-1-Ig detected a band of appr. 39 kDa in WT cell lysate with a faint corresponding band in KD cell lysate. Furthermore, several bands of both higher and lower molecular weight were observed. A band was also detected following IP (2 µg Ab used). This band was more intense than the corresponding band in input extract (4% of starting material). Only a faint band was detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does deplete the antigen from the cell lysate. In IF (1:500 Ab dilution), comparable staining intensities were observed in both KD and WT cells.

We highly encourage other scientists to share their observations.