CSNK1A1, ABclonal, A9308

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Casein kinase I isoform alpha and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 10 commercial research antibodies against human Casein kinase I isoform alpha encoded by CSNK1A1.

The collection of antibodies was tested using HCT 116 WT and CSNK1A1 KD cells in the applications WB (WT and KD cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KD cells) according to YCharOS standard protocols (see reference).

This antibody was tested in several cell types in the application Western blot. The antibody dilution 1:1000 was used in all cell types. Lysates of HCT 116, U-87 MG, DMS 53, HT-1080, HEK293T, U-2 OS, HeLa and HAP1 were prepared and run in a Western blot where A9308 detected a band of appr. 39 kDa in all cell types. No corresponding band was observed in KD cell lysates. No band was detected following IP (2 µg Ab used). In IF (1:800 Ab dilution), a clear intense staining was observed in WT cells with some background staining in KD cells.

We highly encourage other scientists to share their observations.