CSF-1, Aviva Systems Biology, ARP44330

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Macrophage colony-stimulating factor 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Macrophage colony-stimulating factor 1 encoded by the gene CSF-1.

The collection of antibodies was tested using U87-MG WT and CSF-1 KO cells in the applications WB (WT and KO cell lysates/25 µg protein) and IP (WT cell lysate) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) ARP44330 detected a very faint band of appr. 60 kDa in WT cells with no corresponding band in KO cells. A band was detected following IP (2.0 µg Ab used). No band was detected in either input extract (10 % of starting material) or immunodepleted extract (10% of total unbound fraction), showing that the antibody does completely deplete the antigen from the cell lysate.

We highly encourage other scientists to share their observations.