CSF-1, Thermo Fisher Scientific, MA5-15599

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Macrophage colony-stimulating factor 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 14 commercial research antibodies against human Macrophage colony-stimulating factor 1 encoded by the gene CSF-1.

The collection of antibodies was tested using U87-MG WT and CSF-1 KO cells in the applications WB (WT and KO cell lysates/25 µg protein) and IP (WT cell lysate) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) MA5-15599 detected no band. No band was detected following IP (2.0 µg Ab used).

We highly encourage other scientists to share their observations.