Calponin-3, Thermo Fisher Scientific, PA5-84057

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Calponin-3 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human Calponin-3 encoded by the CNN3 gene.

The collection of antibodies was tested using HAP1 WT and CNN3 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) PA5-84057 detected a band of appr. 36 kDa in WT cell lysate, with no corresponding band in KO lysate. A band was also detected following IP (2 µg Ab used). This band was more intense than the corresponding band in input extract (4% of starting material). Band was also detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does not completely deplete the antigen from the cell lysate. In IF (1:100 Ab dilution), a clear intense staining was observed in WT cells with no background staining in KO cells.

We highly encourage other scientists to share their observations.