Calponin-3, ABclonal, A20352

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Calponin-3 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 8 commercial research antibodies against human Calponin-3 encoded by the CNN3 gene.

The collection of antibodies was tested using HAP1 WT and CNN3 KO cells in the applications WB (WT and KO cell lysates/20 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) A20352 detected a band of appr. 36 kDa in WT cell lysate with no corresponding band in KO lysate. Several bands of higher and lower molecular weight were observed. One of the bands observed in both genotypes had stronger staining than target protein. No band was detected following IP (2 µg Ab used). In IF (1:700 Ab dilution), an intense staining of WT cells was observed with some background staining in KO cells.

We highly encourage other scientists to share their observations.