CHCHD10, Thermo Fisher Scientific, MA5-27531

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Coiled-coil-helix-coiled-coil-helix domain-containing protein 10 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing four commercial research antibodies against human Coiled-coil-helix-coiled-coil-helix domain-containing protein 10 encoded by CHCHD10

The collection of antibodies was tested using HAP1 WT and CHCHD10 KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 Ab dilution) MA5-27531 detected a single band of appr. 14 kDa in WT lysate, with no corresponding band in KO lysate. Furthermore, this antibody was tested in HCT116 WT, showing a single intense band af 14 kDa. A band was also detected following IP (1 µg Ab used). This band was more intense than the corresponding band in input extract (10% of starting material). No band was detected in immunodepleted extract (10% of total unbound fraction), showing that the antibody does completely deplete the antigen from the cell lysate. In IF (1:100 Ab dilution) an intense staining of WT cells was observed compared to KO cell staining.

We highly encourage other scientists to share their observations.