CHCHD10, Proteintech, 25671-1-AP

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Coiled-coil-helix-coiled-coil-helix domain-containing protein 10 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing four commercial research antibodies against human Coiled-coil-helix-coiled-coil-helix domain-containing protein 10 encoded by CHCHD10

The collection of antibodies was tested using HAP1 WT and CHCHD10 KO cells in the applications WB (WT and KO cell lysates/50 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 Ab dilution) 25671-1-AP detected a single band of appr. 14 kDa in WT lysate, with no corresponding band in KO lysate. A band of higher molecular band was observed for both genotypes. A band was also detected following IP (1 µg Ab used). This band was more intense than the corresponding band in input extract (10% of starting material). Band was also detected in immunodepleted extract (10% of total unbound fraction), showing that the antibody does not completely deplete the antigen from the cell lysate. In IF (1:300 Ab dilution), an intense staining of WT cells was observed compared to KO cell staining.

We highly encourage other scientists to share their observations.